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Anti-DARPP-32 Antibody (1B13)
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产品编号 TMAC-01115
别名 Protein phosphatase 1 regulatory subunit 1B, Protein phosphatase 1 regulatory inhibitor subunit 1B, Protein phosphatase 1 regulatory (inhibitor) subunit 1B, PPR1B_HUMAN, PPP1R1B, Neuronal phosphoprotein DARPP 32, IPPD, FLJ20940, Dopamine- and cAMP-regulated neuronal phosphoprotein, Dopamine and cAMP regulated phosphoprotein DARPP32, Dopamine and cAMP regulated phosphoprotein DARPP 32, Dopamine and cAMP regulated phosphoprotein, Dopamine and cAMP regulated neuronal phosphoprotein 32, Dopamine and cAMP regulated neuronal phosphoprotein, DARPP32, DARPP 32
Anti-DARPP-32 Antibody (1B13) 是一种 Rabbit 抗体，靶向 DARPP-32。Anti-DARPP-32 Antibody (1B13) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-DARPP-32 Antibody (1B13)
Anti-DARPP-32 Antibody (1B13)
还可以产品编号 TMAC-01115 别名 Protein phosphatase 1 regulatory subunit 1B, Protein phosphatase 1 regulatory inhibitor subunit 1B, Protein phosphatase 1 regulatory (inhibitor) subunit 1B, PPR1B_HUMAN, PPP1R1B, Neuronal phosphoprotein DARPP 32, IPPD, FLJ20940, Dopamine- and cAMP-regulated neuronal phosphoprotein, Dopamine and cAMP regulated phosphoprotein DARPP32, Dopamine and cAMP regulated phosphoprotein DARPP 32, Dopamine and cAMP regulated phosphoprotein, Dopamine and cAMP regulated neuronal phosphoprotein 32, Dopamine and cAMP regulated neuronal phosphoprotein, DARPP32, DARPP 32
Anti-DARPP-32 Antibody (1B13) 是一种 Rabbit 抗体，靶向 DARPP-32。Anti-DARPP-32 Antibody (1B13) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-DARPP-32 Antibody (1B13) is a Rabbit antibody targeting DARPP-32. Anti-DARPP-32 Antibody (1B13) can be used in WB,ICC/IF,IHC,IP,FCM. |
| 别名 | Protein phosphatase 1 regulatory subunit 1B, Protein phosphatase 1 regulatory inhibitor subunit 1B, Protein phosphatase 1 regulatory (inhibitor) subunit 1B, PPR1B_HUMAN, PPP1R1B, Neuronal phosphoprotein DARPP 32, IPPD, FLJ20940, Dopamine- and cAMP-regulated neuronal phosphoprotein, Dopamine and cAMP regulated phosphoprotein DARPP32, Dopamine and cAMP regulated phosphoprotein DARPP 32, Dopamine and cAMP regulated phosphoprotein, Dopamine and cAMP regulated neuronal phosphoprotein 32, Dopamine and cAMP regulated neuronal phosphoprotein, DARPP32, DARPP 32 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of DARPP32 on mouse brain lysates using anti-DARPP32 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DARPP32 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin. 5. ICC staining DARPP32 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining DARPP32 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with DARPP32 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson's disease and drug abuse. DARPP-32 (for dopamine and adenosine 3',5'-monophosphate-regulated phosphoprotein) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 32 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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