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Anti-Cyclin E2 Antibody (2N277)
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产品编号 TMAC-01049
别名 G1/S specific cyclin E2, CyclinE2, CYCE2, CYCE 2, CYC E2, CCNE2 protein, CCNE2, CCNE 2, CCN E2
Anti-Cyclin E2 Antibody (2N277) 是一种 Rabbit 抗体，靶向 Cyclin E2。Anti-Cyclin E2 Antibody (2N277) 可用于 WB,ICC/IF,IHC,FCM。
Anti-Cyclin E2 Antibody (2N277)
Anti-Cyclin E2 Antibody (2N277)
还可以产品编号 TMAC-01049 别名 G1/S specific cyclin E2, CyclinE2, CYCE2, CYCE 2, CYC E2, CCNE2 protein, CCNE2, CCNE 2, CCN E2
Anti-Cyclin E2 Antibody (2N277) 是一种 Rabbit 抗体，靶向 Cyclin E2。Anti-Cyclin E2 Antibody (2N277) 可用于 WB,ICC/IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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产品介绍
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抗原信息
化学信息
| 产品描述 | Anti-Cyclin E2 Antibody (2N277) is a Rabbit antibody targeting Cyclin E2. Anti-Cyclin E2 Antibody (2N277) can be used in WB,ICC/IF,IHC,FCM. |
| 别名 | G1/S specific cyclin E2, CyclinE2, CYCE2, CYCE 2, CYC E2, CCNE2 protein, CCNE2, CCNE 2, CCN E2 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse |
| 验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cyclin E2 antibody. Counter stained with hematoxylin. 2. ICC staining Cyclin E2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Cyclin E2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Cyclin E2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Hela cells with Cyclin E2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. Cyclin E2 is a cyclin E-related protein that specifically interacts with Cdk2 and Cdk3 and with p27 and p21. Cyclin E2 expression peaks at the G1/S phase transition of the cell cycle, in parallel with cyclin E. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 47 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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