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Anti-Cyclin E Antibody (1I860)
还可以产品编号 TMAY-01313
Anti-Cyclin E Antibody (1I860) 是一种 Mouse 抗体，靶向 Cyclin E。Anti-Cyclin E Antibody (1I860) 可用于 FCM,ICC/IF。
Anti-Cyclin E Antibody (1I860)
Anti-Cyclin E Antibody (1I860)
还可以产品编号 TMAY-01313
Anti-Cyclin E Antibody (1I860) 是一种 Mouse 抗体，靶向 Cyclin E。Anti-Cyclin E Antibody (1I860) 可用于 FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Cyclin E Antibody (1I860) is a Mouse antibody targeting Cyclin E. Anti-Cyclin E Antibody (1I860) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG2b |
| 克隆号 | 1I860 |
| 交叉反应 | Human |
| 验证活性 | 1. Flow cytometric analysis of Human CCNE1 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CCNE1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of CCNE1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human CCNE1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to nucleolus. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Cyclin E/CCNE1 Protein |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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