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Anti-Cyclin D3 Antibody (5L448)
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产品编号 TMAC-01043
别名 G1/S-specific cyclin-D3, G1/S specific cyclin D3, G1 S specific cyclin D3, D3 type cyclin, CyclinD3, CCND3_HUMAN, Ccnd3, CCND 3
Anti-Cyclin D3 Antibody (5L448) 是一种 Rabbit 抗体，靶向 Cyclin D3。Anti-Cyclin D3 Antibody (5L448) 可用于 WB,ICC/IF,FCM。
Anti-Cyclin D3 Antibody (5L448)
Anti-Cyclin D3 Antibody (5L448)
还可以产品编号 TMAC-01043 别名 G1/S-specific cyclin-D3, G1/S specific cyclin D3, G1 S specific cyclin D3, D3 type cyclin, CyclinD3, CCND3_HUMAN, Ccnd3, CCND 3
Anti-Cyclin D3 Antibody (5L448) 是一种 Rabbit 抗体，靶向 Cyclin D3。Anti-Cyclin D3 Antibody (5L448) 可用于 WB,ICC/IF,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
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| 产品描述 | Anti-Cyclin D3 Antibody (5L448) is a Rabbit antibody targeting Cyclin D3. Anti-Cyclin D3 Antibody (5L448) can be used in WB,ICC/IF,FCM. |
| 别名 | G1/S-specific cyclin-D3, G1/S specific cyclin D3, G1 S specific cyclin D3, D3 type cyclin, CyclinD3, CCND3_HUMAN, Ccnd3, CCND 3 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse |
| 验证活性 | 1. Western blot analysis of Cyclin D3 on different lysates using anti-Cyclin D3 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: Hela. 2. ICC staining Cyclin D3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Cyclin D3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Cyclin D3 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Hela cells with Cyclin D3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000; ICC/IF: 1:50-200; FCM: 1:10-50 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl-1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 32 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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