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Anti-Cyclin B1 Antibody (5Q476)

货号 TMAB-04824 一键复制产品信息
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Anti-Cyclin B1 Antibody (5Q476) 是一种 Rabbit 抗体，靶向 Cyclin B1。Anti-Cyclin B1 Antibody (5Q476) 可用于 WB,ICC/IF,FCM。

Anti-Cyclin B1 Antibody (5Q476)

一键复制产品信息
Rating icon 还可以
货号 TMAB-04824

Anti-Cyclin B1 Antibody (5Q476) 是一种 Rabbit 抗体，靶向 Cyclin B1。Anti-Cyclin B1 Antibody (5Q476) 可用于 WB,ICC/IF,FCM。

Anti-Cyclin B1 Antibody (5Q476)
规格价格库存数量
50 μL
¥ 1,390
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-Cyclin B1 Antibody (5Q476) is a Rabbit antibody targeting Cyclin B1. Anti-Cyclin B1 Antibody (5Q476) can be used in WB,ICC/IF,FCM.
Ig Type
IgG
克隆号
5Q476
反应种属
Human,Mouse
验证活性
1. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Cyclin B1) monoclonal Antibody, unconjugated (TMAB-04824) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, FITC) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. 25 ug total protein per lane of various lysates (see on figure) probed with Cyclin B1 monoclonal antibody, unconjugated (TMAB-04824) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
应用WBICC/IFFCM
推荐剂量
WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1μg/Test
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome.
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].
抗原信息
免疫原
Recombinant Protein: human Cyclin B1 protein（1-150aa）
抗原种属
Human
基因名称
CCNB1
基因ID
891
蛋白名称
G2/mitotic-specific cyclin-B1
Uniprot ID
P14635
功能
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
化学信息
分子量Theoretical: 48 kDa. Actual: 55 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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