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Anti-Cyclin A1 Antibody (2E766)
还可以产品编号 TMAY-01463
Anti-Cyclin A1 Antibody (2E766) 是一种 Mouse 抗体，靶向 Cyclin A1。Anti-Cyclin A1 Antibody (2E766) 可用于 FCM,ICC/IF。
Anti-Cyclin A1 Antibody (2E766)
Anti-Cyclin A1 Antibody (2E766)
还可以产品编号 TMAY-01463
Anti-Cyclin A1 Antibody (2E766) 是一种 Mouse 抗体，靶向 Cyclin A1。Anti-Cyclin A1 Antibody (2E766) 可用于 FCM,ICC/IF。
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抗原信息
| 产品描述 | Anti-Cyclin A1 Antibody (2E766) is a Mouse antibody targeting Cyclin A1. Anti-Cyclin A1 Antibody (2E766) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG2b |
| 克隆号 | 2E766 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human CCNA1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CCNA1 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus. 2. Flow cytometric analysis of Human CCNA1 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CCNA1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cyclin A1 / CCNA1 (rh Cyclin A1 / CCNA1; TMPY-01401; NP_003905.1; Met1-Gln465). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Cyclin A1 is a member of the highly conserved cyclin family that is characterized by a dramatic periodicity in protein abundance, and belongs to the A-type cyclin subfamily. The mammalian A-type cyclin family consists of two members: cyclin A1 and cyclin A2. Different cyclins exhibit distinct expression. Cyclin A1 is expressed in mice exclusively in the germ cell lineage and high rate of cyclinA1 is found in human testis and certain myeloid leukaemia cells. Cyclin A1 is primarily function in the control of meiosis. It serves as regulator subunits binding to cyclin-dependent kinase 1 (Cdk1) and cyclin-dependent kinase 2 (Cdk2), which give two different kinase activities, one appearing in S phase, the other in G2. Through this, cyclin A1 operate the entry and progression in cell cycle. High frequency of cyclin A1 overexpression has been observed in acute myelocytic leukemias, especially those that are at the promyelocyte and myeloblast stages of development. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Cyclin A1 / CCNA1 protein (TMPY-01401) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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