Anti-CXCR5 Antibody (3I647) is a Rabbit antibody targeting CXCR5. Anti-CXCR5 Antibody (3I647) can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

3I647

Human,Mouse,Rat

1. 25 μg total protein per Lane of various lysates probed with CXCR5 monoclonal antibody, unconjugated (TMAB-00512) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR5 Monoclonal Antibody, Unconjugated (TMAB-00512) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR5 Monoclonal Antibody, Unconjugated (TMAB-00512) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR5 Monoclonal Antibody, Unconjugated (TMAB-00512) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR5 Monoclonal Antibody, Unconjugated (TMAB-00512) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR5 Monoclonal Antibody, Unconjugated (TMAB-00512) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR5 Monoclonal Antibody, Unconjugated (TMAB-00512) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
8. The Molt-4 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-CXCR5 antibody (TMAB-00512): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Monoclonal

A synthesized peptide: human CXCR5

Human

GPCR,Blood,CD,Alpha Chemokine Rec. (CXCR),SARS Coronavirus,GPCR