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Anti-CXADR/CAR Antibody (7D440)

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产品编号 TMAY-01175

别名 HCAR, CXADR, coxsackie virus and adenovirus receptor, CAR4/6, CAR

Anti-CXADR/CAR Antibody (7D440) 是一种 Rabbit 抗体，靶向 CXADR/CAR。Anti-CXADR/CAR Antibody (7D440) 可用于 ELISA(Det),FCM,ICC/IF。

Anti-CXADR/CAR Antibody (7D440)

Anti-CXADR/CAR Antibody (7D440)

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产品编号 TMAY-01175 别名 HCAR, CXADR, coxsackie virus and adenovirus receptor, CAR4/6, CAR

Anti-CXADR/CAR Antibody (7D440) 是一种 Rabbit 抗体，靶向 CXADR/CAR。Anti-CXADR/CAR Antibody (7D440) 可用于 ELISA(Det),FCM,ICC/IF。

规格价格库存数量
20 μL
¥ 780
5日内发货
100 μL
¥ 2,294
5日内发货
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产品介绍

生物活性
产品描述
Anti-CXADR/CAR Antibody (7D440) is a Rabbit antibody targeting CXADR/CAR. Anti-CXADR/CAR Antibody (7D440) can be used in ELISA(Det),FCM,ICC/IF.
别名HCAR, CXADR, coxsackie virus and adenovirus receptor, CAR4/6, CAR
Ig Type
Rabbit IgG
克隆号
7D440
交叉反应
Human
特异性
Human CXADR/CAR
验证活性
1. Immunofluorescence staining of Human CXADR in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CXADR monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.
2. Flow cytometric analysis of Human CXADR expression on HT-29 cells. Cells were stained with purified anti-Human CXADR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
应用ELISA(Det)FCMICC/IF
推荐剂量
ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Det): 1:1000-1:10000
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human CXADR / CAR (rh CXADR / CAR; TMPY-02121; NP_001329.1; Met1-Gly237).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0
研究背景CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 17 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 9% sequence identity with the corresponding regions of rat, human CXADR.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
FITC/PE
可提供抗体种类
3
抗原信息
免疫原
Recombinant Protein: Human CXADR / CAR protein (TMPY-02121)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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