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Anti-CRABP2 Antibody (3D707)
还可以产品编号 TMAY-01745
Anti-CRABP2 Antibody (3D707) 是一种 Rabbit 抗体，靶向 CRABP2。Anti-CRABP2 Antibody (3D707) 可用于 WB,FCM,ICC/IF,IP。
Anti-CRABP2 Antibody (3D707)
Anti-CRABP2 Antibody (3D707)
还可以产品编号 TMAY-01745
Anti-CRABP2 Antibody (3D707) 是一种 Rabbit 抗体，靶向 CRABP2。Anti-CRABP2 Antibody (3D707) 可用于 WB,FCM,ICC/IF,IP。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-CRABP2 Antibody (3D707) is a Rabbit antibody targeting CRABP2. Anti-CRABP2 Antibody (3D707) can be used in WB,FCM,ICC/IF,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 3D707 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human CRABP2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human CRABP2 monoclonal antibody (1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Anti-CRABP2 rabbit monoclonal antibody at 1:500 dilution. -Lane A: 293T Whole Cell Lysate. -Lane B: MCF7 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:16 kDa. -Observed band size:14 kDa. 3. CRABP2 was immunoprecipitated using: -Lane A:0.5 mg 293T Whole Cell Lysate. -Lane B:0.5 mg Raji Whole Cell Lysate -0.5 µL anti-CRABP2 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-CRABP2 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 16 kDa. -Observed band size: 16 kDa. 4. Flow cytometric analysis of Human CRABP2 expression on MCF-7 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CRABP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | WB,FCM,ICC/IF,IP |
| 推荐剂量 | WB: 1:500-1:1000; ICC-IF: 1:100-1:500; FCM: 1:100-1:500; IP: 0.1-0.5 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human CRABP2 (rh CRABP2; TMPY-01549; NP_001869.1; Pro2-Glu138). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Cellular retinoic acid-binding protein 2, also known as Cellular retinoic acid-binding protein II, CRABP-II and CRABP2, is a protein which belongs to thecalycin superfamily and Fatty-acid binding protein (FABP) family. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The predicted amino acid sequences of human CRABP1 and CRABP2 demonstrated a 99.3% and 93.5% identity to mouse CRABP1 and CRABP2, respectively. CRABP2 forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Expression of CRABP2, but not CRABP1 mRNA, was markedly increased (greater than 15-fold) by retinoic acid treatment of fibroblasts cultured from human skin, whereas no significant induction of CRABP2 mRNA was observed in human lung fibroblasts. CRABP2 transports retinoic acid to the nucleus. It regulates the access of retinoic acid to the nuclear retinoic acid receptors. CRABP2 is necessary for elastin induction by All-trans retinoic acid (ATRA) in MRC-5 cells. It is expressed at low levels in emphysema fibroblasts. This alteration in the retinoic acid signalling pathway in lung fibroblasts may contribute to the defect of alveolar repair in human pulmonary emphysema. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | PE |
| 可提供抗体种类 | 2 |
| 免疫原 | Recombinant Human CRABP2 protein (TMPY-01549) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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