- 全部删除
- 您的购物车当前为空
Anti-CNPase Antibody (5Q967) 是一种 Rabbit 抗体,靶向 CNPase。Anti-CNPase Antibody (5Q967) 可用于 WB,ICC,IHC,FCM。
Anti-CNPase Antibody (5Q967) 是一种 Rabbit 抗体,靶向 CNPase。Anti-CNPase Antibody (5Q967) 可用于 WB,ICC,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-CNPase Antibody (5Q967) is a Rabbit antibody targeting CNPase. Anti-CNPase Antibody (5Q967) can be used in WB,ICC,IHC,FCM. |
别名 | CNPase, CNP1, CNP 1, CNP, CN37_HUMAN, 3''-cyclic-nucleotide 3''-phosphodiesterase, 2'3' cyclic nucleotide 3' phosphodiesterase, 2'' |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of CNPase on mouse brain lysates using anti-CNPase antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CNPase antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CNPase antibody. Counter stained with hematoxylin. 4. ICC staining CNPase in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining CNPase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining CNPase in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of SH-SY-5Y cells with CNPase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | 2',3'-cyclic nucleotide-3'-phosphodiesterase (CNPase) is a membrane-bound enzyme that can link tubulin to membranes and may regulate cytoplasmic microtubule distribution. CNPase acts as a microtubule-associated protein by promoting microtubule assembly; this activity resides in the C-terminus of the enzyme. CNPase is firmly associated with tubulin from brain tissue and thyroid cells and can be found at high concentrations in central nervous system myelin and in the outer segments of photoreceptors in the retina. Acute lead intoxication leads to disturbances in CNPase activity and the morphology of myelin. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 48 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
评论内容