Anti-CHAT Antibody (3G910) is a Rabbit antibody targeting CHAT. Anti-CHAT Antibody (3G910) can be used in WB,IP,ICC/IF,IHC,FCM.

IgG

Human,Mouse,Rat

1. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- Choline Acetyltransferase antibody. Counter stained with hematoxylin.
2. Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti- Choline Acetyltransferase antibody. Counter stained with hematoxylin.
3. ICC staining ChoLine Acetyltransferase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Choline Acetyltransferase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Choline Acetyltransferase in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of N2A cells with Choline Acetyltransferase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
7. Lane1 Mouse brain tissue lysate Use the Antibody at 1:2K dilution for 1 hour at room temperature.

WB: 1:1000-2000; IHC: 1:100-200; ICC/IF: 1:50-200; IP: 1:20-50; FCM: 1:20-100

Monoclonal

Unconjugated

A synthesized peptide: human CHAT

human