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Anti-CENPC Antibody (3X906) 是一种 Rabbit 抗体,靶向 CENPC。Anti-CENPC Antibody (3X906) 可用于 WB,ICC,IF,IHC,FCM,IP。
Anti-CENPC Antibody (3X906) 是一种 Rabbit 抗体,靶向 CENPC。Anti-CENPC Antibody (3X906) 可用于 WB,ICC,IF,IHC,FCM,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-CENPC Antibody (3X906) is a Rabbit antibody targeting CENPC. Anti-CENPC Antibody (3X906) can be used in WB,ICC,IF,IHC,FCM,IP. |
别名 | MIF2, Interphase centromere complex protein 7, ICEN7, hcp-4, Centromere protein C 1, Centromere protein C, Centromere autoantigen C1, Centromere autoantigen C, CENPC1, CENPC_HUMAN, CENP-C 1, CENP-C |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of CENPC on K562 cell lysate using anti-CENPC antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. 3. Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. 4. ICC staining CENPC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of K562 cells with CENPC antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; IP: 1:10-50; FCM: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The evolutionarily conserved Centromere Protein-C, CENP-C, is a kinetochore assembly protein. CENP-C is located on the fibers of the kinetochore and constitutes a kinetochore organizing center that tightly associates with DNA. CENP-C is necessary for the formation of a functional centromere, which indicates that CENP-C is important for mitotic progression. In addition, CENP-C is lost from centromeres during herpes simplex virus 1 infection, causing substantial structural changes in the kinetochore, which suggests that the structure of CENP-C is regulated during the cell cycle. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein: human CENPC aa 400-600 |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 107 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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