Anti-CDKN1B Polyclonal Antibody is a Rabbit antibody targeting CDKN1B. Anti-CDKN1B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Sheep)

1. Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit Igg (orange);
Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue),
Dilution: 1:200 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA (green).
2. Tissue/cell: human ovary carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CDKN1B/P27kip1 Polyclonal Antibody, Unconjugated (TMAB-00410) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. CDKN1B (p27Kip1) was immunoprecipitated from mouse kidney tissue with TMAB-00410 at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Goat anti-Rabbit IgG (Heavy Chain specific) was used as secondary antibody at 1:5000 dilution.
Lane 1: mouse kidney tissue lysate 10 µg (Input).
Lane 2: TMAB-00410 IP in mouse kidney tissue lysate.
Lane 3: native rabbit IgG IP in mouse kidney tissue lysate (negative control).
Secondary: Goat anti-Rabbit IgG (Heavy Chain specific), HRP Conjugated, 1:5000
4. Sample:
293t (Human) Cell Lysate at 30 μg
U2os (Human) Cell Lysate at 30 μg
Primary: Anti-CDKN1B/p27 KIP 1 (TMAB-00410) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human P27 kip1

Human

Cip/Kip,Cip / Kip,Cip/kip