Anti-CDK2 Polyclonal Antibody 2 is a Rabbit antibody targeting CDK2. Anti-CDK2 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Pig,Cow,Horse,Rabbit)

1. Blank control (Black line): U87Mg (Black).
Primary Antibody (green line): Rabbit Anti-CDK2 antibody (TMAB-00404)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Sample:
Lane 1: LympHnode (Mouse) Lysate at 40 μg
Lane 2: Esophagus (Mouse) Lysate at 40 μg
Lane 3: K562 (Human) Cell Lysate at 30 μg
Lane 4: Molt-4 (Human) Cell Lysate at 30 μg
Lane 5: Siha (Human) Cell Lysate at 30 μg
Primary:
Anti-CDK2 (TMAB-00404) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa

WB: 1:500-2000; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CDK2

Human

Cdks,Cdks,Cdks