Anti-CDK2 Polyclonal Antibody is a Rabbit antibody targeting CDK2. Anti-CDK2 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CDK2 Polyclonal Antibody, Unconjugated (TMAB-00403) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: K562 Cell (Human) Lysate at 40 μg
Primary: Anti-CDK2 (TMAB-00403) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 35 kDa
3. Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-CDK2 antibody (TMAB-00403)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CDK2

Human

Cdks,Cdks,Cdks