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Anti-CDH11 Antibody (6T438) 是一种 Mouse 抗体,靶向 CDH11。Anti-CDH11 Antibody (6T438) 可用于 WB,ICC,IHC,FCM。
Anti-CDH11 Antibody (6T438) 是一种 Mouse 抗体,靶向 CDH11。Anti-CDH11 Antibody (6T438) 可用于 WB,ICC,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,980 | 5日内发货 | |
100 μL | ¥ 2,990 | 5日内发货 |
产品描述 | Anti-CDH11 Antibody (6T438) is a Mouse antibody targeting CDH11. Anti-CDH11 Antibody (6T438) can be used in WB,ICC,IHC,FCM. |
别名 | Osteoblast cadherin, OSF-4, OSF4, OB-cadherin, OBcadherin, OB, CDHOB, Cdh11, Cadherin 11 type 2 OB cadherin (osteoblast), Cadherin 11 type 2, Cadherin 11, CAD11 |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of CDH11 on human CDH11 recombinant protein using anti-CDH11 antibody at 1/1,000 dilution. 2. Western blot analysis of CDH11 on HEK293 (1) and CDH11-hIgGFc transfected HEK293 (2) cell lysate using anti-CDH11 antibody at 1/1,000 dilution. 3. Western blot analysis of CDH11 on MCF-7 (1), Jurkat (2) and HEK293 (3) cell lysate using anti-CDH11 antibody at 1/1,000 dilution. 4. Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CDH11 antibody. Counter stained with hematoxylin. 5. ICC staining CDH11 (green) and Actin filaments (red) in HL-7702 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Hela cells with CDH11 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Cadherins are calcium dependent cell adhesion proteins, a family of Ca++-dependent adhesion molecules that influence cell-cell binding and are critical to the maintenance of tissue structure and morphogenesis. OB-cadherin (osteoblast-cadherin, cadherin-11, OSF-4) has two forms, OB-cadherin-1 and OB-cadherin-2. OB-cadherin-2 has a truncated cytoplasmic domain, missing amino acids 694-796. Both OB-cadherins are expressed in osteoblastic cell lines with low expression seen in lungs, testis and brain. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 88 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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