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Anti-CDC40 Antibody (4S858)

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Anti-CDC40 Antibody (4S858)
产品编号 TMAC-00769
别名 PRPF17, PRP17_HUMAN, PRP17 homolog, PRP17, Pre-mRNA-processing factor 17, Pre mRNA splicing factor 17, Pre mRNA processing factor 17, hPRP17, EH-binding protein 3, Ehb3, EH binding protein 3, Cell division cycle 40 homolog, Cell division cycle 40, Cdc 40

Anti-CDC40 Antibody (4S858) 是一种 Rabbit 抗体，靶向 CDC40。Anti-CDC40 Antibody (4S858) 可用于 WB,ICC/IF,IHC,FCM。

Anti-CDC40 Antibody (4S858)

Anti-CDC40 Antibody (4S858)

Rating icon 还可以
Anti-CDC40 Antibody (4S858)
产品编号 TMAC-00769 别名 PRPF17, PRP17_HUMAN, PRP17 homolog, PRP17, Pre-mRNA-processing factor 17, Pre mRNA splicing factor 17, Pre mRNA processing factor 17, hPRP17, EH-binding protein 3, Ehb3, EH binding protein 3, Cell division cycle 40 homolog, Cell division cycle 40, Cdc 40

Anti-CDC40 Antibody (4S858) 是一种 Rabbit 抗体，靶向 CDC40。Anti-CDC40 Antibody (4S858) 可用于 WB,ICC/IF,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,495
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-CDC40 Antibody (4S858) is a Rabbit antibody targeting CDC40. Anti-CDC40 Antibody (4S858) can be used in WB,ICC/IF,IHC,FCM.
别名PRPF17, PRP17_HUMAN, PRP17 homolog, PRP17, Pre-mRNA-processing factor 17, Pre mRNA splicing factor 17, Pre mRNA processing factor 17, hPRP17, EH-binding protein 3, Ehb3, EH binding protein 3, Cell division cycle 40 homolog, Cell division cycle 40, Cdc 40
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of CDC40 on Hela cells lysates using anti-CDC40 antibody at 1/2,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CDC40 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CDC40 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CDC40 antibody. Counter stained with hematoxylin.
5. ICC staining CDC40 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining CDC40 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining CDC40 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of K562 cells with CDC40 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
WB
ICC/IF
IHC
FCM
推荐剂量
WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division control (Cdc) genes are required at various points in the cell cycle. Cdc40, also known as pre-mRNA-processing factor 17 (PRPF17) or EH-binding protein 3 (EHB3), is a 579 amino acid nuclear protein. Cdc40 is essential for the catalytic step II of the pre-mRNA splicing process, in which Cdc40 associates with the spliceosome C complex. Cdc40 contains seven WD repeats, which are important in protein-protein interactions. Cdc40 has sequence similarity to the yeast protein Prp17, which is involved in pre-mRNA splicing and cell cycle progression. The sequence similarity between the mammalian Cdc40 and the yeast Prp17 may indicate an additional role in cell cycle progression for mammalian Cdc40.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
O60508
化学信息
分子量Theoretical: 66 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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