Anti-CDC37 Antibody (7U387) is an antibody targeting CDC37. Anti-CDC37 Antibody (7U387) can be used in ELISA, WB, FCM.

Rabbit IgG

7U387

Human

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate
-All lanes: CDC37 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 45 kDa
-Observed band size: 50 kDa
2. Overlay histogram showing Hela cells stained with TMAH-00239 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, FCM

WB:1:500-1:5000; FCM:1:20-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human Cdc37

Human

11140

Epigenetics and Nuclear Signaling, Cell biology