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Anti-CD99 Antibody (5Q457) 是一种 Rabbit 抗体,靶向 CD99。Anti-CD99 Antibody (5Q457) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-CD99 Antibody (5Q457) 是一种 Rabbit 抗体,靶向 CD99。Anti-CD99 Antibody (5Q457) 可用于 WB,ICC/IF,IHC,IP,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-CD99 Antibody (5Q457) is a Rabbit antibody targeting CD99. Anti-CD99 Antibody (5Q457) can be used in WB,ICC/IF,IHC,IP,FCM. |
别名 | T-cell surface glycoprotein E2, Protein MIC2, E2 antigen, CD99 antigen, 120000000 |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of CD99 on THP-1 cells lysates using anti-CD99 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CD99 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD99 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD99 antibody. Counter stained with hematoxylin. 5. ICC staining CD99 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining CD99 in PANC-1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining CD99 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Jurkat cells with CD99 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | MIC2, also designated CD99, is a T cell surface protein that is involved in the aggregation of lymphocytes. Two forms of MIC2, which are differentially expressed, are produced by alternative splicing. The major form induces cellular adhesion, whereas the truncated form inhibits the adhesion process. MIC2 regulates the LFA-1/ICAM-1-mediated adhesion of lymphocytes. Overexpression of the truncated form results in downregulated expression of LFA-1. Cells with downregulated MIC2 exhibit a Hodgkin's and Reed-Sternberg (H-RS) phenotype, indicating that MIC2 plays an important role in regulating cell function and morphology. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 25 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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