Anti-CD86 Polyclonal Antibody is a Rabbit antibody targeting CD86. Anti-CD86 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat

1. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated (TMAB-00396) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 μg
Lane 2: U937 (Human) Cell Lysate at 30 μg
Lane 3: Spleen (Rat) Lysate at 40 μg
Lane 4: Spleen (Mouse) Lysate at 40 μg
Lane 5: HL-60 (Human) Cell Lysate at 30 μg
Primary: Anti-CD86 (TMAB-00396) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72-74 kDa
Observed band size: 72 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: the middle of rat CD86

Rat

CD86

T-lymphocyte activation antigen CD86

Monocytic Lineage,Dendritic Cell Lineage,B Lymphocytic Lineage,Host Virus Interaction,CD markers ELISA kits,Non-lineage,CD,Regulatory T Cells

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.