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Anti-CD86 Antibody (5O340)
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产品编号 TMAC-00739
别名 T-lymphocyte activation antigen CD86, FUN-1, CTLA-4 counter-receptor B7.2, BU63, B70, Activation B7-2 antigen
Anti-CD86 Antibody (5O340) 是一种 Rabbit 抗体，靶向 CD86。Anti-CD86 Antibody (5O340) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-CD86 Antibody (5O340)
Anti-CD86 Antibody (5O340)
还可以产品编号 TMAC-00739 别名 T-lymphocyte activation antigen CD86, FUN-1, CTLA-4 counter-receptor B7.2, BU63, B70, Activation B7-2 antigen
Anti-CD86 Antibody (5O340) 是一种 Rabbit 抗体，靶向 CD86。Anti-CD86 Antibody (5O340) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-CD86 Antibody (5O340) is a Rabbit antibody targeting CD86. Anti-CD86 Antibody (5O340) can be used in WB,ICC/IF,IHC,IP,FCM. |
| 别名 | T-lymphocyte activation antigen CD86, FUN-1, CTLA-4 counter-receptor B7.2, BU63, B70, Activation B7-2 antigen |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of CD86 on different lysates using anti-CD86 antibody at 1/1,000 dilution. Positive control: Lane 1: Raji, Lane 2: Jurkat. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD86 antibody. Counter stained with hematoxylin. 3. ICC staining CD86 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining CD86 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining CD86 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of K562 cells with CD86 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins B7-1 (CD80) and B7-2 (CD86) expressed on antigen presenting cells bind the homologous T cell receptors CD28 and CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28, and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counterreceptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell. |
| 偶联 | Unconjugated |
| 免疫原 | A synthesized peptide: within Human CD86 aa 1-50 / 329 |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Theoretical: 38 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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