Anti-CD80 Polyclonal Antibody 2 is a Rabbit antibody targeting CD80. Anti-CD80 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human,Mouse,Rat

1. Sample:
Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 μg
Lane 2: SP2/0 (Mouse) Cell Lysate at 30 μg
Lane 3: Thymus (Rat) Lysate at 40 μg
Lane 4: Raji (Human) Cell Lysate at 30 μg
Lane 5: K562 (Human) Cell Lysate at 30 μg
Primary: Anti-CD80 (TMAB-00393) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 60 kDa
2. Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CD80 antibody (TMAB-00393), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange),used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-00393, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
3. Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-CD80 antibody (TMAB-00393)
Dilution: 2 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 2µg/Test

Polyclonal

KLH conjugated synthetic peptide: rat CD80

Rat

CD,B Lymphocytic Lineage,Dendritic Cell Lineage,Monocytic Lineage