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Anti-CD68 Antibody (5F511) 是一种 Mouse 抗体,靶向 CD68。Anti-CD68 Antibody (5F511) 可用于 IHC,IF。
Anti-CD68 Antibody (5F511) 是一种 Mouse 抗体,靶向 CD68。Anti-CD68 Antibody (5F511) 可用于 IHC,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,345 | 5日内发货 | |
100 μL | ¥ 2,185 | 5日内发货 |
产品描述 | Anti-CD68 Antibody (5F511) is a Mouse antibody targeting CD68. Anti-CD68 Antibody (5F511) can be used in IHC,IF. |
别名 | SCARD1, LAMP4, GP110, CD68 molecule |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. IHC staining of paraffin-embedded Human tonsil tissue CD68 mouse mAb diluted at 1:200. 2. Immunofluorescence analysis of Human-lung-cancer tissue. 1,CD68 Monoclonal Antibody (red) was diluted at 1:200 (4℃, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B. 3. Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,CD68 Monoclonal Antibody was diluted at 1:200(4℃, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only. 4. Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,CD68 Monoclonal Antibody was diluted at 1:200(4℃,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. 5. Immunofluorescence analysis of Mouse-spleen tissue. 1,CD68 Monoclonal Antibody (red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B。 |
应用 | |
推荐剂量 | IHC: 1:200; IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | Affinity purification using immunogen. |
性状 | Liquid |
缓冲液 | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. |
偶联 | Unconjugated |
Uniprot ID |
分子量 | Actual: 37 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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