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Anti-CD64 Antibody (8F637)
还可以产品编号 TMAY-03088
Anti-CD64 Antibody (8F637) 是一种 Rabbit 抗体，靶向 CD64。Anti-CD64 Antibody (8F637) 可用于 FCM,ICC/IF。
Anti-CD64 Antibody (8F637)
Anti-CD64 Antibody (8F637)
还可以产品编号 TMAY-03088
Anti-CD64 Antibody (8F637) 是一种 Rabbit 抗体，靶向 CD64。Anti-CD64 Antibody (8F637) 可用于 FCM,ICC/IF。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-CD64 Antibody (8F637) is a Rabbit antibody targeting CD64. Anti-CD64 Antibody (8F637) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 8F637 |
| 交叉反应 | Mouse |
| 验证活性 | 1. Flow cytometric analysis of Mouse FCGR1(CD64) expression on Raw264.7 cells. Cells were stained with purified anti-Mouse FCGR1(CD64), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of mouse FCGR1 in Raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse FCGR1 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD64 / FCGR1 (rM CD64 / FCGR1; TMPY-01205; NP_034316.1; Met1-Pro297). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/PerCP/FITC/PE |
| 可提供抗体种类 | 5 |
| 免疫原 | Recombinant Mouse CD64 / FCGR1 protein (TMPY-01205) |
| 抗原种属 | Mouse |
| 研究领域 | Fc Receptors |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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