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Anti-CD63 Antibody (4R240)
还可以产品编号 TMAY-01811
Anti-CD63 Antibody (4R240) 是一种 Mouse 抗体，靶向 CD63。Anti-CD63 Antibody (4R240) 可用于 FCM,ICC/IF。
Anti-CD63 Antibody (4R240)
Anti-CD63 Antibody (4R240)
还可以产品编号 TMAY-01811
Anti-CD63 Antibody (4R240) 是一种 Mouse 抗体，靶向 CD63。Anti-CD63 Antibody (4R240) 可用于 FCM,ICC/IF。
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偶联与修饰
抗原信息
| 产品描述 | Anti-CD63 Antibody (4R240) is a Mouse antibody targeting CD63. Anti-CD63 Antibody (4R240) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 4R240 |
| 交叉反应 | Human |
| 验证活性 | 1. Flow cytometric analysis of Human CD63 expression on MCF-7 cells. Cells were stained with purified anti-Human CD63, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of CD63 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human CD63 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD63 / Tspan-30 / Tetraspanin-30 (rh CD63 / Tspan-30 / Tetraspanin-30; TMPY-02099; P08962-1; Ala103-Val203). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 63 (CD63) is a member of the CD family and the transmembrane 4 superfamily, also known as the tetraspanin family. CD63 is a cell surface glycoprotein characterized by the presence of four hydrophobic domains. CD63 had functions in mediating signal transduction processes and then regulate a variety of cellular processes such as cell proliferation, activation and motility. It has been reported that CD63 protein associated with tumor progression and served as a blood platelet activation marker and the deficiency of this protein may be associated with Hermansky-Pudlak syndrome. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/FITC/PE |
| 可提供抗体种类 | 4 |
| 免疫原 | Recombinant Human CD63 / Tspan-30 / Tetraspanin-30 protein (TMPY-02099) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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