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Anti-CD34 Antibody (1D438)
还可以产品编号 TMAY-03369
Anti-CD34 Antibody (1D438) 是一种 Rabbit 抗体，靶向 CD34。Anti-CD34 Antibody (1D438) 可用于 ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP。
Anti-CD34 Antibody (1D438)
Anti-CD34 Antibody (1D438)
还可以产品编号 TMAY-03369
Anti-CD34 Antibody (1D438) 是一种 Rabbit 抗体，靶向 CD34。Anti-CD34 Antibody (1D438) 可用于 ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-CD34 Antibody (1D438) is a Rabbit antibody targeting CD34. Anti-CD34 Antibody (1D438) can be used in ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 1D438 |
| 交叉反应 | Mouse |
| 验证活性 | 1. Immunochemical Staining of mouse CD34 in mouse stomach with Rabbit Monoclonal Antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to vascular endothelial cells. 2. Immunochemical Staining of mouse CD34 in mouse kidney with Rabbit Monoclonal Antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to vascular endothelial cells. 3. Flow cytometric analysis of CD34 expression on M1 cells. Cells were stained with purified anti-Mouse CD34, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 4. Mouse CD34 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg 293T Whole Cell Lysate -0.5 µL anti-Mouse CD34 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Mouse CD34 rabbit monoclonal antibody, at 1:350 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 41 kDa. -Observed band size: 130 kDa. 5. Immunofluorescence staining of CD34 in M1 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD34 monoclonal antibody (1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). |
| 应用 | ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP |
| 推荐剂量 | ELISA: 1:25000-1:50000; IHC-P: 1:2000-1:10000; ICC-IF: 1:100-1:500; FCM: 1:100-1:500; IP: 0.2-1 μL/mg of lysate; ELISA(Det): 1:5000-1:50000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD34 (rM CD34; TMPY-01652; NP_001104529.1; Met 1-Thr 287). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/PerCP/FITC/PE |
| 可提供抗体种类 | 5 |
| 免疫原 | Recombinant Mouse CD34 Protein (TMPY-01652) |
| 抗原种属 | Mouse |
| 研究领域 | Cardiac Stem Cell Markers, Hemangioblast Markers |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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