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Anti-CD31/PECAM-1 Antibody (8O85)
还可以产品编号 TMAY-00348
Anti-CD31/PECAM-1 Antibody (8O85) 是一种 Mouse 抗体，靶向 CD31/PECAM-1。Anti-CD31/PECAM-1 Antibody (8O85) 可用于 FCM,ICC/IF。
Anti-CD31/PECAM-1 Antibody (8O85)
Anti-CD31/PECAM-1 Antibody (8O85)
还可以产品编号 TMAY-00348
Anti-CD31/PECAM-1 Antibody (8O85) 是一种 Mouse 抗体，靶向 CD31/PECAM-1。Anti-CD31/PECAM-1 Antibody (8O85) 可用于 FCM,ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 1,689 | 5日内发货 |
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抗原信息
| 产品描述 | Anti-CD31/PECAM-1 Antibody (8O85) is a Mouse antibody targeting CD31/PECAM-1. Anti-CD31/PECAM-1 Antibody (8O85) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 8O85 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human CD31 in HUVEC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD31 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody. Positive staining was localized to cell membrane. 2. Flow cytometric analysis of anti-human CD31 on human whole blood monocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable monocytes. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD31 / PECAM1 extracellular domain (rhCD31; TMPY-00970; Met 1-Lys 601; EAW94208.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/FITC/PE |
| 可提供抗体种类 | 4 |
| 免疫原 | Recombinant Human CD31 protein (TMPY-00970) |
| 抗原种属 | Human |
| 研究领域 | Hemangioblast Markers, ITIM/ITAM Immunoreceptors and Related Molecules |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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