Anti-CD28 Polyclonal Antibody is a Rabbit antibody targeting CD28. Anti-CD28 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Cow,Horse)

1. Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated (TMAB-00349) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated (TMAB-00349) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Positive control: (mo) splenocytes (2% Paraformaldehyde-fixed)
Isotype Control Antibody
Antibody: Rabbit IgG;
Supplier: Cell Signaling Technology;
Dilution: 1 μg in 100 μL 1 X PBS containing 0.5% BSA
Secondary Antibody
Antibody: Goat anti-rabbit IgG-FITC;
Supplier: Cell Signaling Technology;
Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody
Supplier: Bioss;
Supplier catalog number: TMAB-00349;
Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA
4. Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD28) Polyclonal Antibody, Unconjugated (TMAB-00349) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: mouse CD28

Mouse

CD,Regulatory T Cells,CD,NK Cell Lineage,T Lymphocytic Lineage