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Anti-CD166/ALCAM Antibody (3O275)
还可以产品编号 TMAY-00159
Anti-CD166/ALCAM Antibody (3O275) 是一种 Mouse 抗体，靶向 CD166/ALCAM。Anti-CD166/ALCAM Antibody (3O275) 可用于 FCM,ICC/IF。
Anti-CD166/ALCAM Antibody (3O275)
Anti-CD166/ALCAM Antibody (3O275)
还可以产品编号 TMAY-00159
Anti-CD166/ALCAM Antibody (3O275) 是一种 Mouse 抗体，靶向 CD166/ALCAM。Anti-CD166/ALCAM Antibody (3O275) 可用于 FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-CD166/ALCAM Antibody (3O275) is a Mouse antibody targeting CD166/ALCAM. Anti-CD166/ALCAM Antibody (3O275) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 3O275 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human CD166 in MSC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD166 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane. 2. Flow cytometric analysis of human ALCAM(CD166) expression on Jurkat cells. Cells were stained with purified anti-Human ALCAM(CD166), then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD166 / ALCAM (rh CD166 / ALCAM; TMPY-01578; NP_001618.2; Met1-Ala526). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/FITC/PE |
| 可提供抗体种类 | 4 |
| 免疫原 | Recombinant Human CD166 / ALCAM protein (TMPY-01578) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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