Anti-CD14 Antibody (7C57)

Name: Anti-CD14 Antibody (7C57)
Brand: TargetMol
SKU: TMAH-00167
Rating: 4.5 (1 reviews)

还可以

产品编号 TMAH-00167

别名 monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen

Anti-CD14 Antibody (7C57) 是一种 Mouse 抗体，靶向 CD14。Anti-CD14 Antibody (7C57) 可用于 ELISA, WB, IHC, IF。

Anti-CD14 Antibody (7C57)

还可以

产品编号 TMAH-00167 别名 monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen

Anti-CD14 Antibody (7C57) 是一种 Mouse 抗体，靶向 CD14。Anti-CD14 Antibody (7C57) 可用于 ELISA, WB, IHC, IF。

规格	价格	库存	数量
50 μL	¥ 1,305	5日内发货
100 μL	¥ 2,190	5日内发货

大包装 & 定制

加入购物车

TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。

实验操作小课堂

常见问题解答

联系我们获取更多批次信息

资源下载

产品操作手册

产品介绍

生物活性

产品描述	Anti-CD14 Antibody (7C57) is a Mouse antibody targeting CD14. Anti-CD14 Antibody (7C57) can be used in ELISA, WB, IHC, IF.
别名	monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen
Ig Type	IgG1
克隆号	7C57
交叉反应	Human, Mouse, Rat, Rabbit
验证活性	1. Western Blot -Positive WB detected in: Rabbit lung tissue, Rabbit liver tissue, Rabbit spleen tissue, Rabbit small intestine tissue -All lanes: CD14 antibody at 1:2500 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 41 kDa -Observed band size: 55 kDa 2. Western Blot -Positive WB detected in: Mouse kidney tissue, Rat liver tissue -All lanes: CD14 antibody at 1:2000 SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 41 kDa -Observed band size: 55 kDa 3. Western Blot -Positive WB detected in: NIH/3T3 whole cell lysate -All lanes: CD14 antibody at at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 41 kDa -Observed band size: 55 kDa 4. Western Blot -Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate -All lanes: CD14 antibody at 1:1000 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 41 kDa -Observed band size: 55 kDa 5. Immunofluorescence staining of A549 cells with TMAH-00167 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). 6. Immunofluorescence staining of HepG2 cells with TMAH-00167 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). 7. Immunofluorescence staining of Raw264.7 cells with TMAH-00167 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
应用	ELISA, WB, IHC, IF
抗体种类	Monoclonal
宿主来源	Mouse
亚细胞定位	Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Membrane raft. Golgi apparatus.
构建方式	Hybridoma Monoclonal Antibody
纯化方式	Protein A purified
性状	Liquid
缓冲液	Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
纯度	>95%
研究背景	Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-).

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	Recombinant Protein: Human Monocyte differentiation antigen CD14 Protein (20-345AA)
抗原种属	Human
基因ID	929
Uniprot ID	P08571
研究领域	Immunology