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顶部产品介绍偶联与修饰抗原信息储存和溶解信息计算器

Anti-CD14 Antibody (7C57)

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货号 TMAH-00167

别名 monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen

Anti-CD14 Antibody (7C57) 是一种 Mouse 抗体，靶向 CD14。Anti-CD14 Antibody (7C57) 可用于 ELISA,IF,IHC,WB。

Anti-CD14 Antibody (7C57)

Anti-CD14 Antibody (7C57)

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货号 TMAH-00167 别名 monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen

Anti-CD14 Antibody (7C57) 是一种 Mouse 抗体，靶向 CD14。Anti-CD14 Antibody (7C57) 可用于 ELISA,IF,IHC,WB。

规格价格库存数量
50 μL
¥ 1,305
5日内发货
100 μL
¥ 2,190
5日内发货
库存状态实时更新，以官网显示为准，现货产品可直接加购物车下单
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产品介绍

生物活性
产品描述
Anti-CD14 Antibody (7C57) is a Mouse antibody targeting CD14. Anti-CD14 Antibody (7C57) can be used in ELISA,IF,IHC,WB.
别名monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen
Ig Type
IgG1
克隆号
7C57
反应种属
Human, Mouse, Rat, Rabbit
验证活性
1. Western Blot
-Positive WB detected in: Rabbit lung tissue, Rabbit liver tissue, Rabbit spleen tissue, Rabbit small intestine tissue
-All lanes: CD14 antibody at 1:2500
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 41 kDa
-Observed band size: 55 kDa
2. Western Blot
-Positive WB detected in: Mouse kidney tissue, Rat liver tissue
-All lanes: CD14 antibody at 1:2000
SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 41 kDa
-Observed band size: 55 kDa
3. Western Blot
-Positive WB detected in: NIH/3T3 whole cell lysate
-All lanes: CD14 antibody at at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 41 kDa
-Observed band size: 55 kDa
4. Western Blot
-Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate
-All lanes: CD14 antibody at 1:1000
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 41 kDa
-Observed band size: 55 kDa
5. Immunofluorescence staining of A549 cells with TMAH-00167 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
6. Immunofluorescence staining of HepG2 cells with TMAH-00167 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
7. Immunofluorescence staining of Raw264.7 cells with TMAH-00167 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
应用ELISAIFIHCWB
抗体种类
Monoclonal
宿主来源Mouse
亚细胞定位Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Membrane raft. Golgi apparatus.
组织特异性Detected on macrophages (at protein level). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
构建方式Hybridoma Monoclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
纯度>95%
研究背景Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-).
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human Monocyte differentiation antigen CD14 Protein (20-345AA)
抗原种属
Human
基因名称
CD14
基因ID
929
Uniprot ID
P08571
研究领域
Immunology
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | -20°C for 1 year

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