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Anti-CBX5 Antibody (5G758) 是一种 Rabbit 抗体,靶向 CBX5。Anti-CBX5 Antibody (5G758) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-CBX5 Antibody (5G758) 是一种 Rabbit 抗体,靶向 CBX5。Anti-CBX5 Antibody (5G758) 可用于 WB,ICC/IF,IHC,IP,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-CBX5 Antibody (5G758) is a Rabbit antibody targeting CBX5. Anti-CBX5 Antibody (5G758) can be used in WB,ICC/IF,IHC,IP,FCM. |
别名 | HP1A, Heterochromatin protein 1 homolog alpha (HP1 alpha), Chromobox protein homolog 5, CBX5, Antigen p25 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of HP1 alpha on different cell lysates using anti-HP1 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7, Lane 2: Hela. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin. 6. ICC staining HP1 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining HP1 alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of SH-SY-5Y cells with HP1 alpha antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The family of Heterochromatin Protein 1 (HP1) ("Chromobox Homolog", CBX) are highly conserved adapter proteins, which have important functions in the cell nucleus. These functions include gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.Three different paralogs of HP1 are found in mammals, HP1α, HP1βand HP1γ. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 22 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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