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顶部生物活性储存和溶解信息计算器

Anti-Caspase-14 Antibody (3A777)

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Anti-Caspase-14 Antibody (3A777)
产品编号 TMAC-00578
别名 caspase 14, apoptosis-related cysteine peptidase, ARCI12

Anti-Caspase-14 Antibody (3A777) 是一种 Rabbit 抗体，靶向 Caspase-14。Anti-Caspase-14 Antibody (3A777) 可用于 WB,ICC/IF,IHC,IP,FCM。

Anti-Caspase-14 Antibody (3A777)

Anti-Caspase-14 Antibody (3A777)

Rating icon 还可以
Anti-Caspase-14 Antibody (3A777)
产品编号 TMAC-00578 别名 caspase 14, apoptosis-related cysteine peptidase, ARCI12

Anti-Caspase-14 Antibody (3A777) 是一种 Rabbit 抗体，靶向 Caspase-14。Anti-Caspase-14 Antibody (3A777) 可用于 WB,ICC/IF,IHC,IP,FCM。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-Caspase-14 Antibody (3A777) is a Rabbit antibody targeting Caspase-14. Anti-Caspase-14 Antibody (3A777) can be used in WB,ICC/IF,IHC,IP,FCM.
别名caspase 14, apoptosis-related cysteine peptidase, ARCI12
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of Caspase-14 on MCF-7 cells lysates using anti-Caspase-14 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Caspase-14 antibody. Counter stained with hematoxylin.
3. ICC staining Caspase-14 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Caspase-14 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Caspase-14 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of A431 cells with Caspase-14 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
WB
ICC/IF
IHC
IP
FCM
推荐剂量
WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is composed of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6 and caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9, caspase-10, and caspase-14. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. Caspase-14, also designated MICE (for mini-ICE), is highly expressed in embryonic tissues but appears to be absent from adult tissues. Procaspase-14 can be processed in vitro by caspase-8 and caspase-10 but not by other caspases.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P31944
化学信息
分子量Theoretical: 28 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

ARCI-12ARCI 12

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