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Anti-CAMKIV Antibody (5K734)
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产品编号 TMAC-00550
别名 D18Bwg0362e, CaMKIV/Gr, CaMKIV, calcium/calmodulin-dependent protein kinase IV, AI666733, A430110E23Rik
Anti-CAMKIV Antibody (5K734) 是一种 Rabbit 抗体，靶向 CAMKIV。Anti-CAMKIV Antibody (5K734) 可用于 WB,ICC,IF,IHC,FCM。
Anti-CAMKIV Antibody (5K734)
Anti-CAMKIV Antibody (5K734)
还可以产品编号 TMAC-00550 别名 D18Bwg0362e, CaMKIV/Gr, CaMKIV, calcium/calmodulin-dependent protein kinase IV, AI666733, A430110E23Rik
Anti-CAMKIV Antibody (5K734) 是一种 Rabbit 抗体，靶向 CAMKIV。Anti-CAMKIV Antibody (5K734) 可用于 WB,ICC,IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-CAMKIV Antibody (5K734) is a Rabbit antibody targeting CAMKIV. Anti-CAMKIV Antibody (5K734) can be used in WB,ICC,IF,IHC,FCM. |
| 别名 | D18Bwg0362e, CaMKIV/Gr, CaMKIV, calcium/calmodulin-dependent protein kinase IV, AI666733, A430110E23Rik |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of CAMKIV on mouse brain and rat brain lysates using anti-CAMKIV antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CAMKIV antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CAMKIV antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CAMKIV antibody. Counter stained with hematoxylin. 5. ICC staining CAMKIV in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining CAMKIV in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining CAMKIV in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of SH-SY-5Y cells with CAMKIV antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: C-terminus human CAMKIV |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Theoretical: 52 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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