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Anti-Calreticulin Antibody (3C852)

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Anti-Calreticulin Antibody (3C852)
产品编号 TMAB-00274
别名 SSA, RO, HEL-S-99n, CRT, cC1qR, calreticulin

Anti-Calreticulin Antibody (3C852) 是一种 Rabbit 抗体,靶向 Calreticulin。Anti-Calreticulin Antibody (3C852) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM。

Anti-Calreticulin Antibody (3C852)

Anti-Calreticulin Antibody (3C852)

Rating icon 还可以
Anti-Calreticulin Antibody (3C852)
产品编号 TMAB-00274 别名 SSA, RO, HEL-S-99n, CRT, cC1qR, calreticulin

Anti-Calreticulin Antibody (3C852) 是一种 Rabbit 抗体,靶向 Calreticulin。Anti-Calreticulin Antibody (3C852) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM。

规格价格库存数量
25 μL
¥ 785
5日内发货
50 μL
¥ 1,395
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-Calreticulin Antibody (3C852) is a Rabbit antibody targeting Calreticulin. Anti-Calreticulin Antibody (3C852) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
别名SSA, RO, HEL-S-99n, CRT, cC1qR, calreticulin
Ig Type
IgG
克隆号
3C852
交叉反应
Human,Mouse,Rat
验证活性
1. 25 μg total protein per Lane of various lysates probed with Calreticulin monoclonal antibody, unconjugated (TMAB-00274) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
16. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
17. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Monoclonal Antibody, Unconjugated (TMAB-00274) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:20-50
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface.
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300.
研究背景Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020]
抗原信息
免疫原
A synthesized peptide: human Calreticulin
抗原种属
Human
基因ID
Uniprot ID
研究领域
ER
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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