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Anti-CALML5 Antibody (2A846)

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Anti-CALML5 Antibody (2A846)
产品编号 TMAY-02056
别名 CLSP, calmodulin like 5

Anti-CALML5 Antibody (2A846) 是一种 Rabbit 抗体，靶向 CALML5。Anti-CALML5 Antibody (2A846) 可用于 ELISA,FCM,ICC/IF。

Anti-CALML5 Antibody (2A846)

Anti-CALML5 Antibody (2A846)

Rating icon 还可以
Anti-CALML5 Antibody (2A846)
产品编号 TMAY-02056 别名 CLSP, calmodulin like 5

Anti-CALML5 Antibody (2A846) 是一种 Rabbit 抗体，靶向 CALML5。Anti-CALML5 Antibody (2A846) 可用于 ELISA,FCM,ICC/IF。

规格价格库存数量
20 μL
¥ 780
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100 μL
¥ 2,289
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产品介绍

生物活性
产品描述
Anti-CALML5 Antibody (2A846) is a Rabbit antibody targeting CALML5. Anti-CALML5 Antibody (2A846) can be used in ELISA,FCM,ICC/IF.
别名CLSP, calmodulin like 5
Ig Type
Rabbit IgG
克隆号
2A846
交叉反应
Human
验证活性
1. Immunofluorescence staining of Human CALML5 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CALML5 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
2. Flow cytometric analysis of Human CALML5 expression on A431 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CALML5, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
应用
ELISA
FCM
ICC/IF
推荐剂量
ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human CALML5 (rh CALML5; TMPY-01953; AAH39172.1; Met 1-Glu 146).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Calmodulin-like protein 5, also known as Calmodulin-like skin protein, CALML5 and CLSP, is a protein which contains fourEF-hand domains. CALML5 / CLSP is particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation.The expression is very low in lung. CALML5 / CLSP binds calcium. It may be involved in terminal differentiation of keratinocytes. Coxsackievirus and adenovirus receptor (CAR) is a member of the immunoglobulin (Ig) superfamily and a component of epithelial tight junction. CAR functions as a primary receptor for coxsackievirus B and adenovirus (Ad) infection. CALML5 / CLSP is closely related to CAR. The structure and dynamics of human calmodulin-like skin protein CALML5 / CLSP have been characterized by NMR spectroscopy. The mobility of CALML5 / CLSP has been found to be different for the N-terminal and C-terminal domains. The N-terminal domain is characterized by four stable helices, which experience large fluctuations. This is shown to be due to mutations in the hydrophobic core. The overall N-terminal domain behavior is similar both in the full-length protein and in the isolated domain.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human CALML5 protein (TMPY-01953)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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