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Anti-Caldesmon Antibody (6J397)
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产品编号 TMAC-00523
别名 NAG22, MGC21352, LCAD, L CAD, HCAD, H CAD, CDM, Caldesmon1, Caldesmon 1 Isoform 5, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 1, Caldesmon 1, Caldesmon, CALD1_HUMAN, CALD1, CALD 1, CAD
Anti-Caldesmon Antibody (6J397) 是一种 Rabbit 抗体，靶向 Caldesmon。Anti-Caldesmon Antibody (6J397) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-Caldesmon Antibody (6J397)
Anti-Caldesmon Antibody (6J397)
还可以产品编号 TMAC-00523 别名 NAG22, MGC21352, LCAD, L CAD, HCAD, H CAD, CDM, Caldesmon1, Caldesmon 1 Isoform 5, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 1, Caldesmon 1, Caldesmon, CALD1_HUMAN, CALD1, CALD 1, CAD
Anti-Caldesmon Antibody (6J397) 是一种 Rabbit 抗体，靶向 Caldesmon。Anti-Caldesmon Antibody (6J397) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-Caldesmon Antibody (6J397) is a Rabbit antibody targeting Caldesmon. Anti-Caldesmon Antibody (6J397) can be used in WB,ICC/IF,IHC,IP,FCM. |
| 别名 | NAG22, MGC21352, LCAD, L CAD, HCAD, H CAD, CDM, Caldesmon1, Caldesmon 1 Isoform 5, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 1, Caldesmon 1, Caldesmon, CALD1_HUMAN, CALD1, CALD 1, CAD |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Caldesmon on NIH/3T3 cell lysates using anti-Caldesmon antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Caldesmon antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin. 5. ICC staining Caldesmon in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Caldesmon in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of NIH/3T3 cells with Caldesmon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Caldesmon, Filamin 1, Nebulin and Villin are differentially expressed and regulated Actin binding proteins. Both muscular and non-muscular forms of Caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. Alternative splicing of the gene encoding Caldesmon results in five isoforms. Muscular Caldesmon (isoform 1), also designated high molecular weight Caldesmon or H-Caldesmon (H-CAD), is expressed predominantly on thin filaments in smooth muscle. Non-muscular Caldesmon (isoforms 2-5), also designated low molecular weight Caldesmon or L-Caldesmon (L-CAD), is widely expressed in non-muscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of Nebulin are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 70 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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