Anti-C5a Polyclonal Antibody is a Rabbit antibody targeting C5a. Anti-C5a Polyclonal Antibody can be used in FCM,WB.

IgG

Human (predicted:Mouse,Rat)

1. Sample: HepG2 (human) Lysate at 40 μg
Primary: Anti-C5 (TMAB-00262) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 104/112 kDa
Observed band size: 117 kDa
2. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-C5 antibody (TMAB-00262)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample:
Lane 1: A549 (Human) Cell Lysate at 30 μg
Lane 2: Hela (Human) Cell Lysate at 30 μg
Primary: Anti-C5a (TMAB-00262) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115/105 kDa
Observed band size: 115 kDa

WB: 1:500-2000; FCM: 2ug/Test

Polyclonal

KLH conjugated synthetic peptide: human Complement C5 alpha chain

Human

C5

Complement C5

Oxidative Stress,Alternative Pathway,Classical Pathway,MAC,Macrophage / Inflamm.,Oxidative stress,GPCR

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).