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Anti-C12orf51 Antibody (8V885) 是一种 Mouse 抗体,靶向 C12orf51。Anti-C12orf51 Antibody (8V885) 可用于 WB,IHC,ICC,FCM。
Anti-C12orf51 Antibody (8V885) 是一种 Mouse 抗体,靶向 C12orf51。Anti-C12orf51 Antibody (8V885) 可用于 WB,IHC,ICC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,995 | 5日内发货 | |
100 μL | ¥ 2,995 | 5日内发货 |
产品描述 | Anti-C12orf51 Antibody (8V885) is a Mouse antibody targeting C12orf51. Anti-C12orf51 Antibody (8V885) can be used in WB,IHC,ICC,FCM. |
别名 | Probable E3 ubiquitin-protein ligase HECTD4, KIAA0614, HECTD4, HECT domain-containing protein 4, C12orf51 |
交叉反应 | Human,Mouse |
验证活性 | 1. Western blot analysis of C12orf51 on SH-SY5Y (1) and A549 (2) using anti-C12orf51 antibody at 1/100 dilution. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-C12orf51 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-C12orf51 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-C12orf51 antibody. Counter stained with hematoxylin. 5. ICC staining C12orf51 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining C12orf51 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of SH-SY5Y cells with C12orf51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
应用 | |
推荐剂量 | WB: 1:500; IHC: 1:50-100; ICC: 1:50; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 438/145/98/262 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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