Anti-BTN2A1 Polyclonal Antibody is a Rabbit antibody targeting BTN2A1. Anti-BTN2A1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human

1. Blank control: SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-BTN2A1 antibody (TMAB-00255)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Blank control: MCF7 cells (blue). Primary Antibody: Rabbit Anti-BTN2A1 antibody (TMAB-00255), Dilution: 5 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange),used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
3. Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (BTN2A1) Polyclonal Antibody, Unconjugated (TMAB-00255) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: human BTN2A1

Human

Lipid metabolism,Channels