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Anti-BRD2 Antibody (7Q375) 是一种 Rabbit 抗体,靶向 BRD2。Anti-BRD2 Antibody (7Q375) 可用于 WB,ICC,IHC,FCM。
Anti-BRD2 Antibody (7Q375) 是一种 Rabbit 抗体,靶向 BRD2。Anti-BRD2 Antibody (7Q375) 可用于 WB,ICC,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-BRD2 Antibody (7Q375) is a Rabbit antibody targeting BRD2. Anti-BRD2 Antibody (7Q375) can be used in WB,ICC,IHC,FCM. |
别名 | RNF3, RING3 PROTEIN, RING3, Really interesting new gene 3 protein, O27.1.1, NAT, KIAA9001, FSRG1, FSH, FLJ31942, Female sterile homeotic related gene 1, mouse, homolog of, Female sterile homeotic related gene 1, DKFZp686N0336, D6S113E, Bromodomain-containing protein 2, Bromodomain containing 2, BRD2_HUMAN, Brd2, BRD 2 |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of BRD2 on SiHa cell lysate using anti-BRD2 antibody at 1/2,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BRD2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BRD2 antibody. Counter stained with hematoxylin. 4. ICC staining BRD2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining BRD2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining BRD2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of 293T cells with BRD2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The bromodomain-containing proteins include BRD2, BRD3, BRD4 and BRDT. BRD2 (RING3 protein) is a mitogen-activated nuclear protein whose gene is located in the human MHC II region, suggesting its relation to HLA-associated diseases. The gene encoding BRD3 (RING3-like protein) contains two bromodomains and maps to chromosome 9q34. BRD4 (HUNK1 protein) is a nuclear protein involved in the regulation of chromosomal dynamics during mitosis. The testis-specific bromodomain protein BRDT contains a PEST sequence, indicating that it undergoes rapid intracellular degradation. The bromodomain-containing proteins are ubiquitously expressed. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein: human BRD2 aa 1-200 |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 88 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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