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Anti-BMP-2 Antibody (1M274)
还可以产品编号 TMAY-00808
Anti-BMP-2 Antibody (1M274) 是一种 Mouse 抗体，靶向 BMP-2。Anti-BMP-2 Antibody (1M274) 可用于 FCM,ICC/IF。
Anti-BMP-2 Antibody (1M274)
Anti-BMP-2 Antibody (1M274)
还可以产品编号 TMAY-00808
Anti-BMP-2 Antibody (1M274) 是一种 Mouse 抗体，靶向 BMP-2。Anti-BMP-2 Antibody (1M274) 可用于 FCM,ICC/IF。
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产品介绍
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抗原信息
| 产品描述 | Anti-BMP-2 Antibody (1M274) is a Mouse antibody targeting BMP-2. Anti-BMP-2 Antibody (1M274) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 1M274 |
| 交叉反应 | Human, Mouse, Rat, Rhesus, Canine |
| 验证活性 | 1. Immunofluorescence staining of Human BMP2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human BMP2 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human BMP2 expression on Hela cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human BMP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human / Mouse / Rat / Rhesus / Canine BMP-2 (rh BMP-2; TMPY-01136; NP_001191.1; Gln283-Arg396). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human / Mouse / Rat / Rhesus / Canine BMP-2 protein (TMPY-01136) |
| 抗原种属 | Human, Mouse, Rat, Rhesus, Canine |
| 研究领域 | Early Mesodermal Lineage Markers |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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