Anti-BID Antibody (9U548)

Name: Anti-BID Antibody (9U548)
Brand: TargetMol
SKU: TMAY-00869
Rating: 4.5 (1 reviews)

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产品编号 TMAY-00869

Anti-BID Antibody (9U548) 是一种 Mouse 抗体，靶向 BID。Anti-BID Antibody (9U548) 可用于 WB,ELISA,ELISA(Cap),FCM,ICC/IF。

Anti-BID Antibody (9U548)

还可以

产品编号 TMAY-00869

Anti-BID Antibody (9U548) 是一种 Mouse 抗体，靶向 BID。Anti-BID Antibody (9U548) 可用于 WB,ELISA,ELISA(Cap),FCM,ICC/IF。

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生物活性

产品描述	Anti-BID Antibody (9U548) is a Mouse antibody targeting BID. Anti-BID Antibody (9U548) can be used in WB,ELISA,ELISA(Cap),FCM,ICC/IF.
Ig Type	Monoclonal Mouse IgG1
克隆号	9U548
交叉反应	Human
验证活性	1. Immunofluorescence staining of Human BID in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human BID monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm. 2. Human BID expression in Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-BID, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 3. Anti-BID mouse monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:22 kDa. -Observed band size:22 kDa
应用	WB,ELISA,ELISA(Cap),FCM,ICC/IF
推荐剂量	WB: 1:500-1:2000; ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000
抗体种类	Monoclonal
宿主来源	Mouse
构建方式	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BID (rh BID; TMPY-02027; P55957-1; Met 1-Asp 195). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
纯化方式	Protein A
性状	Liquid
缓冲液	0.2 μm filtered solution in PBS
研究背景	The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.

偶联与修饰

偶联	Unconjugated
其他可选修饰符	FITC
可提供抗体种类	2

抗原信息

免疫原	Recombinant Human BID protein (TMPY-02027)
抗原种属	Human

存储&运输

储存方式	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式	Shipping with blue ice.