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Anti-Beta-Actin Antibody (1R828)
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产品编号 TMAC-00427
别名 β-Actin Protein, β-actin, Beta-Actin, Actin Cytoplasmic 1, ACTB
Anti-Beta-Actin Antibody (1R828) 是一种 Mouse 抗体，靶向 Beta-Actin。Anti-Beta-Actin Antibody (1R828) 可用于 WB,ICC,IHC,FCM。
Anti-Beta-Actin Antibody (1R828)
Anti-Beta-Actin Antibody (1R828)
还可以产品编号 TMAC-00427 别名 β-Actin Protein, β-actin, Beta-Actin, Actin Cytoplasmic 1, ACTB
Anti-Beta-Actin Antibody (1R828) 是一种 Mouse 抗体，靶向 Beta-Actin。Anti-Beta-Actin Antibody (1R828) 可用于 WB,ICC,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,995 | 5日内发货 | |
| 100 μL | ¥ 2,985 | 5日内发货 |
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| 产品描述 | Anti-Beta-Actin Antibody (1R828) is a Mouse antibody targeting Beta-Actin. Anti-Beta-Actin Antibody (1R828) can be used in WB,ICC,IHC,FCM. |
| 别名 | β-Actin Protein, β-actin, Beta-Actin, Actin Cytoplasmic 1, ACTB |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Beta-Western blot analysis of Beta-actin on different lysates using anti-Beta-actin antibody at 1/10,000 dilution. Positivecontrol: Lane 1: NIH/3T3, Lane 2: PC12, Lane 3: MCF-7, Lane 4: HepG2, Lane 5: Hela, Lane 6: Mouse lung. 2. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta-actin antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Beta-actin antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beta-actin antibody. Counter stained with hematoxylin. 5. ICC staining of Beta-actin in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining of Beta-actin in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining of Beta-actin in NIH-3T3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela?cells with Beta-actin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:10000; IHC: 1:100-200; ICC: 1:100-200; FCM: 1:100-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Beta actin also know as Cytoplasmic Actin is a 42 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error. |
| 偶联 | Unconjugated |
| 免疫原 | KLH conjugated synthetic peptide: within human Beta-actin N terminal |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Theoretical: 42 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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