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顶部生物活性储存和溶解信息计算器

Anti-Beta-Actin Antibody (1R828)

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产品编号 TMAC-00427

别名 β-Actin Protein, β-actin, Beta-Actin, Actin Cytoplasmic 1, ACTB

Anti-Beta-Actin Antibody (1R828) 是一种 Mouse 抗体，靶向 Beta-Actin。Anti-Beta-Actin Antibody (1R828) 可用于 FCM,ICC,IHC,WB。

Anti-Beta-Actin Antibody (1R828)

Anti-Beta-Actin Antibody (1R828)

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Rating icon 还可以
产品编号 TMAC-00427 别名 β-Actin Protein, β-actin, Beta-Actin, Actin Cytoplasmic 1, ACTB

Anti-Beta-Actin Antibody (1R828) 是一种 Mouse 抗体，靶向 Beta-Actin。Anti-Beta-Actin Antibody (1R828) 可用于 FCM,ICC,IHC,WB。

规格价格库存数量
50 μL
¥ 1,995
5日内发货
100 μL
¥ 2,985
5日内发货
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产品介绍

生物活性
产品描述
Anti-Beta-Actin Antibody (1R828) is a Mouse antibody targeting Beta-Actin. Anti-Beta-Actin Antibody (1R828) can be used in FCM,ICC,IHC,WB.
别名β-Actin Protein, β-actin, Beta-Actin, Actin Cytoplasmic 1, ACTB
克隆号
1R828
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of Beta-Western blot analysis of Beta-actin on different lysates using anti-Beta-actin antibody at 1/10,000 dilution. Positivecontrol: Lane 1: NIH/3T3, Lane 2: PC12, Lane 3: MCF-7, Lane 4: HepG2, Lane 5: Hela, Lane 6: Mouse lung.
2. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
5. ICC staining of Beta-actin in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining of Beta-actin in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining of Beta-actin in NIH-3T3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of Hela?cells with Beta-actin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
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应用FCMICCIHCWB
推荐剂量
WB: 1:10000; IHC: 1:100-200; ICC: 1:100-200; FCM: 1:100-200
抗体种类
Monoclonal
宿主来源Mouse
构建方式Hybridoma Monoclonal Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Beta actin also know as Cytoplasmic Actin is a 42 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
KLH conjugated synthetic peptide: within human Beta-actin N terminal
抗原种属
human
Uniprot ID
P60709
化学信息
分子量Theoretical: 42 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

b-Actin

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