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Anti-BCL2 Antibody (9E869)
还可以产品编号 TMAH-00107
别名 PPP1R50, Bcl-2, B-cell CLL/lymphoma 2
Anti-BCL2 Antibody (9E869) 是一种 Mouse 抗体，靶向 BCL2。Anti-BCL2 Antibody (9E869) 可用于 ELISA, WB, FC。
Anti-BCL2 Antibody (9E869)
Anti-BCL2 Antibody (9E869)
还可以产品编号 TMAH-00107 别名 PPP1R50, Bcl-2, B-cell CLL/lymphoma 2
Anti-BCL2 Antibody (9E869) 是一种 Mouse 抗体，靶向 BCL2。Anti-BCL2 Antibody (9E869) 可用于 ELISA, WB, FC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-BCL2 Antibody (9E869) is a Mouse antibody targeting BCL2. Anti-BCL2 Antibody (9E869) can be used in ELISA, WB, FC. |
| 别名 | PPP1R50, Bcl-2, B-cell CLL/lymphoma 2 |
| Ig Type | IgG1 |
| 克隆号 | 9E869 |
| 交叉反应 | Human, Mouse |
| 验证活性 | 1. Western Blot -Positive WB detected in: BCL2 antibody at 1:1000 -Lane 1: Hela whole cell lysate -Lane 2: THP-1 whole cell lysate -Lane 3: NIH/3T3 whole cell lysate -Lane 4: MCF-7 whole cell lysate -Secondary: Goat polyclonal to Mouse IgG at 1/20000 dilution -Predicted band size: 26KDa -Observed band size: 26 KDa -Exposure time: 5min 2. Overlay Peak curve showing Jurkat cells stained with TMAH-00107 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | ELISA, WB, FC |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| 纯度 | >95% |
| 研究背景 | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function. May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: Human poptosis regulator Bcl-2 Protein (2-211AA) |
| 抗原种属 | Human |
| 基因ID | 596 |
| Uniprot ID | |
| 研究领域 | Cell Biology |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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