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Anti-BCL-XL Antibody (8X993)
还可以产品编号 TMAY-00851
Anti-BCL-XL Antibody (8X993) 是一种 Rabbit 抗体，靶向 BCL-XL。Anti-BCL-XL Antibody (8X993) 可用于 WB,ELISA,IP。
Anti-BCL-XL Antibody (8X993)
Anti-BCL-XL Antibody (8X993)
还可以产品编号 TMAY-00851
Anti-BCL-XL Antibody (8X993) 是一种 Rabbit 抗体，靶向 BCL-XL。Anti-BCL-XL Antibody (8X993) 可用于 WB,ELISA,IP。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-BCL-XL Antibody (8X993) is a Rabbit antibody targeting BCL-XL. Anti-BCL-XL Antibody (8X993) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 8X993 |
| 交叉反应 | Human |
| 验证活性 | 1. BCL2L1 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg K562 Whole Cell Lysate. -1 µL anti-BCL2L1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-BCL2L1 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 26 kDa. -Observed band size: 26 kDa. 2. Anti-BCL2L1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: K562 Whole Cell Lysate. -Lane C: NIH-3T3 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:30 kDa |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IP: 0.2-1 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human BCL2L1 / Bcl-XL (rh BCL2L1 / Bcl-XL; TMPY-02431; NP_612815.1; Met1-Arg212). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1), belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is characterized as an important factor in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human BCL2L1/Bcl-XL Protein (TMPY-02431) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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