Anti-BAK1 Antibody (5Y66)

Name: Anti-BAK1 Antibody (5Y66)
Brand: TargetMol
SKU: TMAH-00103
Rating: 4.5 (1 reviews)

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产品编号 TMAH-00103

别名 CDN1, Bcl-2-like protein 7, Bcl2-L-7, BCL2L7, Bcl-2 homologous antagonist/killer, BAK 1, BAK, Apoptosis regulator BAK

Anti-BAK1 Antibody (5Y66) 是一种抗体，靶向 BAK1。Anti-BAK1 Antibody (5Y66) 可用于 ELISA, WB, IHC, FC, IP。

Anti-BAK1 Antibody (5Y66)

还可以

产品编号 TMAH-00103 别名 CDN1, Bcl-2-like protein 7, Bcl2-L-7, BCL2L7, Bcl-2 homologous antagonist/killer, BAK 1, BAK, Apoptosis regulator BAK

Anti-BAK1 Antibody (5Y66) 是一种抗体，靶向 BAK1。Anti-BAK1 Antibody (5Y66) 可用于 ELISA, WB, IHC, FC, IP。

规格	价格	库存	数量
50 μL	¥ 1,300	5日内发货
100 μL	¥ 2,185	5日内发货

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产品描述	Anti-BAK1 Antibody (5Y66) is an antibody targeting BAK1. Anti-BAK1 Antibody (5Y66) can be used in ELISA, WB, IHC, FC, IP.
别名	CDN1, Bcl-2-like protein 7, Bcl2-L-7, BCL2L7, Bcl-2 homologous antagonist/killer, BAK 1, BAK, Apoptosis regulator BAK
Ig Type	Rabbit IgG
克隆号	5Y66
交叉反应	Human
验证活性	1. Western Blot -Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, Colo320 whole cell lysate -All lanes: BAK1 antibody at 0.9μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 24, 17 KDa -Observed band size: 24 KDa 2. IHC image of TMAH-00103 diluted at 1:90 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 3. IHC image of TMAH-00103 diluted at 1:90 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 4. Immunoprecipitating BAK1 in HEK293 whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00103 in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00103 (3μg) + HEK293 whole cell lysate (500μg) -Lane 3: HEK293 whole cell lysate (20μg) 5. Overlay histogram showing Hela cells stained with TMAH-00103 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
应用	ELISA, WB, IHC, FC, IP
推荐剂量	WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000.
抗体种类	Monoclonal
亚细胞定位	Mitochondrion outer membrane; Single-pass membrane protein.
构建方式	Recombinant Antibody
纯化方式	Affinity-chromatography
性状	Liquid
缓冲液	Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景	Plays a role in the mitochondrial apoptosic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.