Anti-BAFF/TNFSF13B Antibody (9F115) is a Rabbit antibody targeting BAFF/TNFSF13B. Anti-BAFF/TNFSF13B Antibody (9F115) can be used in WB,FCM,IP.

Monoclonal Rabbit IgG

9F115

Human

1. Flow cytometric analysis of Human BAFF(CD257) expression on U937 cells. Cells were stained with purified anti-Human BAFF(CD257), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

2. Anti-BAFF rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HL-60 Whole Cell Lysate.

-Lane B: Jurkat Whole Cell Lysate.

-Lane C: MOLT-4 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:34 kDa.

-Observed band size:32 kDa(We are unsure as to the identity of these extra bands.)

3. BAFF was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate.

-Lane B:0.5 mg Jurkat Whole Cell Lysate.

-2 µL anti-BAFF rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-BAFF rabbit monoclonal antibody, at 1:200 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 31 kDa.

-Observed band size: 31 kDa

WB,FCM,IP

WB: 1:500-1:2000; FCM: 1:25-1:100; IP: 1-4 μL/mg of lysate

Monoclonal

Unconjugated

PE

2

Recombinant Human BLyS / TNFSF13B / BAFF protein (TMPY-02775)

Human