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Anti-B2M/beta 2-Microglobulin Antibody (9P155)
还可以产品编号 TMAY-02180
Anti-B2M/beta 2-Microglobulin Antibody (9P155) 是一种 Mouse 抗体，靶向 B2M/beta 2-Microglobulin。Anti-B2M/beta 2-Microglobulin Antibody (9P155) 可用于 WB,ELISA,FCM,ICC/IF。
Anti-B2M/beta 2-Microglobulin Antibody (9P155)
Anti-B2M/beta 2-Microglobulin Antibody (9P155)
还可以产品编号 TMAY-02180
Anti-B2M/beta 2-Microglobulin Antibody (9P155) 是一种 Mouse 抗体，靶向 B2M/beta 2-Microglobulin。Anti-B2M/beta 2-Microglobulin Antibody (9P155) 可用于 WB,ELISA,FCM,ICC/IF。
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偶联与修饰
抗原信息
| 产品描述 | Anti-B2M/beta 2-Microglobulin Antibody (9P155) is a Mouse antibody targeting B2M/beta 2-Microglobulin. Anti-B2M/beta 2-Microglobulin Antibody (9P155) can be used in WB,ELISA,FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 9P155 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-B2M mouse monoclonal antibody at 1:500 dilution. -Lane A: A-431 Whole Cell Lysate. -Lane B: U-937 Whole Cell Lysate. -Lane C: Raji Whole Cell Lysate. -Lane D: HeLa Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:13 kDa. -Observed band size:13 kDa. 2. Immunofluorescence staining of B2M in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human B2M monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor?488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm. 3. Flow cytometric analysis of Human B2M / Beta-2-microglobulin expression on human whole blood lymphocytes. Cells were stained with purified anti-Human B2M / Beta-2-microglobulin, then a PE-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. |
| 应用 | WB,ELISA,FCM,ICC/IF |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Beta-2 microglobulin/B2M (rh Beta-2 microglobulin/B2M; TMPY-01686; NP_004039.1; Met1-Met119). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | B2M, also known as β2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens. B2M (β2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to the α3 chain. Unlike α3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M (β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (β2 microglobulin) can indicate non-progression of HIV. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/FITC/PE |
| 可提供抗体种类 | 4 |
| 免疫原 | Recombinant Human Beta-2 microglobulin/B2M Protein (TMPY-01686) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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