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Anti-ATP7B Antibody (7M703)
还可以产品编号 TMAH-00095
别名 WND/140 kDa, WND, Wilson disease associated protein, WD, WC1, tx, Toxic milk, PWD, Cu++ transporting, Cu(2+) transporting, Copper transporting ATPase 2, Copper pump 2, beta polypeptide, ATPase, ATP7B
Anti-ATP7B Antibody (7M703) 是一种抗体，靶向 ATP7B。Anti-ATP7B Antibody (7M703) 可用于 ELISA, IF, FC。
Anti-ATP7B Antibody (7M703)
Anti-ATP7B Antibody (7M703)
还可以产品编号 TMAH-00095 别名 WND/140 kDa, WND, Wilson disease associated protein, WD, WC1, tx, Toxic milk, PWD, Cu++ transporting, Cu(2+) transporting, Copper transporting ATPase 2, Copper pump 2, beta polypeptide, ATPase, ATP7B
Anti-ATP7B Antibody (7M703) 是一种抗体，靶向 ATP7B。Anti-ATP7B Antibody (7M703) 可用于 ELISA, IF, FC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-ATP7B Antibody (7M703) is an antibody targeting ATP7B. Anti-ATP7B Antibody (7M703) can be used in ELISA, IF, FC. |
| 别名 | WND/140 kDa, WND, Wilson disease associated protein, WD, WC1, tx, Toxic milk, PWD, Cu++ transporting, Cu(2+) transporting, Copper transporting ATPase 2, Copper pump 2, beta polypeptide, ATPase, ATP7B |
| Ig Type | Rabbit IgG |
| 克隆号 | 7M703 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Hela cell with TMAH-00095 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 2. Overlay Peak curve showing Hela cells stained with TMAH-00095 (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | ELISA, IF, FC |
| 推荐剂量 | IF:1:50-1:200; FC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Late endosome.; [Isoform 1]: Golgi apparatus membrane; Multi-pass membrane protein.; [Isoform 2]: Cytoplasm.; [WND/140 kDa]: Mitochondrion. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| 研究背景 | Copper ion transmembrane transporter involved in the export of copper out of the cells. It is involved in copper homeostasis in the liver, where it ensures the efflux of copper from hepatocytes into the bile in response to copper overload. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: Human ATP7B Protein |
| 抗原种属 | Human |
| 基因ID | 540 |
| Uniprot ID | |
| 研究领域 | Metabolism, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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