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顶部生物活性储存和溶解信息计算器

Anti-ATF-4 Antibody (7M603)

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Anti-ATF-4 Antibody (7M603)
产品编号 TMAC-00314

Anti-ATF-4 Antibody (7M603) 是一种 Rabbit 抗体，靶向 ATF-4。Anti-ATF-4 Antibody (7M603) 可用于 WB,ICC/IF,IHC,IP,FCM。

Anti-ATF-4 Antibody (7M603)

Anti-ATF-4 Antibody (7M603)

Rating icon 还可以
Anti-ATF-4 Antibody (7M603)
产品编号 TMAC-00314

Anti-ATF-4 Antibody (7M603) 是一种 Rabbit 抗体，靶向 ATF-4。Anti-ATF-4 Antibody (7M603) 可用于 WB,ICC/IF,IHC,IP,FCM。

规格价格库存数量
50 μL
¥ 1,495
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-ATF-4 Antibody (7M603) is a Rabbit antibody targeting ATF-4. Anti-ATF-4 Antibody (7M603) can be used in WB,ICC/IF,IHC,IP,FCM.
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of ATF4 on different lysates using anti-ATF4 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: PC-12.
2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ATF4 antibody. Counter stained with hematoxylin.
3. ICC staining ATF4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining ATF4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining ATF4 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of Hela cells with ATF4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
WB
ICC/IF
IHC
IP
FCM
推荐剂量
WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promoter and enhancer elements. The cAMP response element (CRE), one of the best studied of such elements, consists of the palindromic octanucleotide TGACGTCA. Several CRE binding proteins have been identified within the ATF/CREB family, the best characterized of which include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. These proteins share highly related COOH terminal leucine zipper dimerization and basic DNA binding domains but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins appear capable of binding CRE in its homodimeric form, certain of these also bind as heterodimers, both within the ATF/CREB family and even with members of the AP-1 transcription factor family.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P18848
化学信息
分子量Theoretical: 55 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

rATF-4rATF4Cyclic AMP-dependent transcription factor ATF-4cAMP-dependent transcription factor ATF-4Atf4Atf 4Activating transcription factor 4 (rATF-4)

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